GT-T551培养基适合人T淋巴细胞（T细胞）的扩增。培养基添加了人白蛋白（医药级别），重组人胰岛素，L-谷氨酰胺和链霉素，不含其它蛋白和生长因子。不论是否添加血清，GT-T551都能支持细胞稳定生长。

GT-T551培养基支持Anti-CD3/IL-2介导的T细胞刺激活化。用这种方法激活T细胞时，如添加RetroNectin重组人纤维蛋白片段可以增强T细胞的扩增，而且所得T细胞比单独使用CD3单抗产生更多 naïve T cells（初始T细胞）。

GT-T551 培养基产品特点

日本原装进口无血清培养基，已经加入人血白蛋白组分。

支持淋巴细胞的高密度细胞培养。

在日本多家医院用于临床 的细胞培养。

配制用水符合国家药监局《人体细胞 研究和制剂质量控制指导原则》中注射用水标准。

GT-T551 培养液在使用前加入0.6%自体血浆，性能可以和许多高价培养液效果相同。

GT-T551 H3 是GT-T551的升级版培养基，采用全新的缓冲系统，可用于无血浆培养，CD3+CD56+比例高。如果培养时添加自体血浆，效果更为显著。

T细胞扩增方法详见TAKARA GT-T551说明书。

GT-T551 is a culture medium ideally suited for expansion of human T lymphocytes (T cells). It is supplemented with human albumin (pharmaceutical grade), recombinant human insulin, L-glutamine, and streptomycin; no additional proteins or growth factors are added. The medium can support stable growth in the presence or absence of serum.

Anti-CD3/IL-2 T-cell activation can also be performed in this medium (Wang et al. 2013; Ai et al. 2014; Dodo et al. 2014). When using this activation method, the additional presence of RetroNectin enhances the proliferation of T cells (Yu et al. 2008; Ishikawa et al. 2014; Hosoi et al. 2014; Sakamoto et al. 2015; Li et al. 2015) and results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone (Hosoi et al. 2014).

Features

Used for extended culture or expansion of T cells

Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin

Endotoxin tested (endotoxin <0.3 EU/ml)

Compatible with CultiLife Culture Bags for large-scale expansion of T cells

Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin enhances proliferation and leads to a higher proportion of naïve T cells. For more information, the user manual can be found under the Documents tab.

The method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research.