Agilent 210515点突变试剂盒

上海华雅思创生物科技有限公司 021-64933729

定点突变：在DNA片段的特定位点引入碱基改变，或片段的插入以及删除。

?1.QuikChangeLighting定点突变

?2.QuikChangeLighting 多点定点突变

QuikChange Lighting单点优势

?快速：反应时间缩短一半

?专利配方的QuikChange Lightning 高保真、高扩增能力的聚合酶配比混合物，避免引入不希望的错误突变

?更高效Dpn I 酶将消化时间从1小时缩短为5分钟，3小时内完成整个突变

?精准：延续了QuikChange II 产品的精确性

?高效：>85%的突变效率

?灵活: 可扩增突变4 到14 kb范围的片段

?宽泛：可用于任何的dam+阳性来源的质粒载体

?可靠：超过15,000论文发表,见证了其*的结果与可靠性

QuikChange Lighting多点优势

?Stratagene*：      ?可一次引入1-5 突变区           ?质粒<8 kb

?专利配方的QuikChange Lightning 高保真、高扩增能力的聚合酶配比混合物

?引物设计:

?每个突变区只需要一条引物  ?所有的突变引物结合同一方向

?扩增出封闭的单链环状产物  ?无插入或缺失突变，只有点突变

?高效:?1-2个突变，效率>90%     ?5个突变，效率大约30%

    美国Stratagene公司是世界生命科学研究领域致力于发展新产品和新技术的*。产品包括：文库构建试剂盒，预制文库，Quik-Change定点突变，双杂交系统，腺病毒表达系统，高灵敏度特异性QPCR试剂，超高保真Pfu酶，基因/蛋白功能分析，支原体检测等。
    包括分子生物学、遗传学、蛋白质组学、药物研发和毒理学等在内的Stratagene产品遍及学术界、政府研究机构和医药工业研究领域，特别是应用于生命科学研究领域的试剂、仪器尤为*。例如文库构建、定点突变、高保真Pfu酶等，部分经典产品说明书更成为被誉为生命科学研究圣地的冷泉港实验室实验课程的标准参考。该公司还提供一系列诊断产品，包括过敏诊断和尿分析等产品。
订购信息：

产品货号 产品描述 主要特性 规格
210518推荐 QuikChange Lightning Mutagenesis Kit  单点突变 质粒<14kb 10T
210519推荐 QuikChange Lightning Mutagenesis Kit  单点突变   30T
200518 QuikChange Site-Directed Mutagenesis Kit  单点突变 质粒<8kb 30T
200519 QuikChange Site-Directed Mutagenesis Kit  单点突变   10T
200523 QuikChange II Site-Directed Mutagenesis Kit  单点突变   10T
200524 QuikChange II Site-Directed Mutagenesis Kit  单点突变   30T
200516 QuikChange XL Site-Directed Mutagenesis Kit  单点突变 质粒>8kb 30T
200517 QuikChange XL Site-Directed Mutagenesis Kit  单点突变   10T
200521 QuikChange II XL Site-Directed Mutagenesis Kit  单点突变   10T
200522 QuikChange II XL Site-Directed Mutagenesis Kit  单点突变   30T
210515推荐 QuikChange Lightning Multi Site-Directed Mutagenesis Kit  多点突变 多达5个点 10T
210513推荐 QuikChange Lightning Multi Site-Directed Mutagenesis Kit  多点突变   30T
200514 QuikChange Multi Site-Directed Mutagenesis Kit  多点突变   30T
200515 QuikChange Multi Site-Directed Mutagenesis Kit  多点突变   10T
200550 GeneMorph II Random Mutagenesis Kit  随机突变 随机突变克隆 10T
200552 GeneMorph II EZClone Domain Mutagenesis Kit  随机突变 趋错随机突变 10T
500402  Dpn I Restriction Enzyme  Dpn I 限制性内切酶 配套试剂 200U

Stratagene公司随机突变试剂盒

   随机突变是指目的基因发生突变的部位是随机的, 如PCR克隆基因时, 往往产生不确定序列的突变。在Tag酶的作用下, 有时在遇到模板中的A-T对时, 倾向于掺入G-C对, 而使基因序列发生改变。随机突变常用于鉴定克隆的DNA中特定功能在基因序列中所处的位置及其与周围序列的关系。如果对所克隆的DNA特定序列所编码的产物及其功能知之甚少，可采用随机突变法进行研究，以确定功能区域有助于缩小研究范围。
Stratagene公司提供如下两种随机突变试剂，介绍如下：
?GeneMorph II Random Mutagenesis Kit
–用于随机突变一段靶序列Mutazyme II DNA 聚合酶：
–Mutazyme I + Taq2000
ü–高错误率的DNA聚合酶混合物
ü–提供均一的突变谱
?GeneMorph II EZClone Domain Mutagenesis Kit
–用于随机突变一段靶序列并可克隆到载体一种反应缓冲体系
ü–专为PCR 反应优化
ü–*突变片断< 6kb
ü–只需改变模板浓度或扩增循环数，即可实现期望的突变频率
安捷伦试剂盒

货号 品名
200049 G418 Sterile Solution,10 ml,50 mg/ml
200123 TG1 Electro-Comp Cells 5 x 0.1 ml
200124 TKX1 Competent Cells 5 x 0.2 ml
200129 XL1-Red Competent Cells 5 x 0.2 ml
200130 XL1-Blue Subclon Grade Comp Cell 8x0.5ml
200131 BL21(DE3) Competent Cells 5 x 0.2 ml
200132 BL21(DE3) pLysS Comp Cells 5 x 0.2 ml
200133 BL21 Competent Cells 5 x 0.2 ml
200134 TKB1 Competent Cells 5 x 0.2 ml
200138 XL-1 Blue MRF' Kan Libr Pack Comp Cells
200150 XL2-Blue UltraComp Cells 10 x 0.1 ml
200151 XL2-Blue MRF UltraComp Cells 10 x 0.1ml
200152 SURE 2 SuperComp Cells 10 x 0.1 ml
200154 BJ5183 Electroporation-Competent Cells
200157 BJ5183-AD-1 Electroporation Comp Cells
200158 XL1-Blue MRF Electro-Comp Cells 5x0.1ml
200159 ElectroTen-Blue Electro-Comp cells
200160 Able Electro-Comp Cells 5 x 0.1 ml
200161 Able C Electro-Comp Cells 5 x 0.1 ml
200162 Able K Electro-Comp Cells 5 x 0.1 ml
200170 Able Comp Cells(Able C and K) 5 x 0.2ml
200171 Able C Competent Cells 5 x 0.2 ml
200172 Able K Competent Cells 5 x 0.2 ml
200201 Gigapack III Gold Packaging Extract,4rxn
200202 Gigapack III Gold Packaging Extract,7rxn
200203 Gigapack III Gold Packaging Extract11rxn
200204 Gigapack III Plus Packaging Extract,4rxn
200205 Gigapack III Plus Packaging Extract,7rxn
200206 Gigapack III Plus Packaging Extract11rxn
200207 Gigapack III XL Packaging Extract, 4 rxn
200208 Gigapack III XL Packaging Extract, 7 rxn
200209 Gigapack III XL Packaging Extract,11 rxn
200220 Transpack Packaging Extract, 400 rxn
200221 Transpack Packaging Extract, 100 rxn
200223 Transpack Packaging Extract, 50 rxn
200227 SURE Electro-Comp Cells 5 x 0.1 ml
200228 XL1-Blue Electro-Comp Cells 5 x 0.1 ml
200229 XL1-Blue MR SuperComp Cells 5 x 0.2 ml
200230 XL1-Blue MRF SuperComp Cells 5 x 0.2 ml
200231 SCSI SuperCompetent Cells 5 x 0.2 ml
200232 AG1 Competent Cells 5 x 0.2 ml
200233 NM522 Competent Cells 5 x 0.2 ml
200234 JM101 Competent Cells 5 x 0.2 ml
200235 JM109 Competent Cells 5 x 0.2 ml
200236 XL1-Blue SuperCompetent Cells 5 x 0.2 ml
200238 SURE Competent Cells 5 x 0.2 ml
200239 JM110 Competent Cells 5 x 0.2 ml
200247 SCS110 Competent Cells 5 x 0.2 ml
200248 XL1-Blue MRF Kan SuperComp Cell 5x0.2ml
200249 XL1-Blue Competent Cells 5 x 0.2 ml
200251 VCSM13 Interference Resistant Hlpr Phg
200252 R408 Interference Resistant Helper Phage
200253 ExAssist Helper Phage w/ SOLR
200285 Mammalian Transfection Kit
200314 XL10-Gold Competent Cells, 5 x 100 ul
200315 XL10-Gold Competent Cells, 10 x 100 ul
200317 XL10-Gold Kanr Comp Cells 10 x 0.1 ml
200324 96Pack Gold Competent Cells
200339 RNAMaxx High Yield Transcription Kit
200344 Kit,Micro RNA Extraction
200345 RNA Isolation Kit
200385 Mammalian Transfection-Calcium Phosphate
200388 Transfection MBS Mammalian Kit
200391 Kit,Lambda DNA Purification,25 miniprep
200392 Kit,Lambda DNA Purfication,50 minipreps
200399 G418 Sulfate, 1 g
200409 PCR Polishing Kit
200410 Klenow Fill-In Kit
200415 Deoxynucleotide Mix, PCR Grad
200435 10x Taq DNA Polymerase Buffer, 10 ml
200436 AffinityScript MltiTempcDNASynthKt,50rxn
200488 ViraPack Transfection Reagent
200513 Kit,QuikChange Multi Site-Directed 30rx
200514 Kt,QuikChange MultiSite-Directed,30rx
200515 QuikChange Multi Site-Drctd Mtgns,10 rx
200516 Kit,QuikChange XL Site-Directed 30 rxn
200517 Kit,QuikChange XL Site-Directed 10 rxn
200518 Kit,QuikChange Site-Directed 30 rxn
200519 Kit,QuikChange Site-Directed 10 rxn
200521 Kit,QuikChange II XL site-directed 10rx
200522 Kit,QuikChange II XL site-directed 30rx
200523 Kit,QuikChange II site-directed 10 rxn
200524 Kit,QuikChange II site-directed 30 rxn
200531 QuikChange MultiSite-DrctdMtgns,10 Rxn,
200532 10x Cloned Pfu DNA Polymerase Buffr,10ml
200550 GeneMorph II random mutagenesis kit
200552 GeneMorph II EZClone Domain Mutagnsis Kt
200555 QuikChange II-E(Electrprtn)StDrctd,10Rx
200600 Kit,DNA Extraction
200820 AccuScript HighFidlty1stStrndcDNASynthKt
201100 PhiX174/Hae III Marker, 20 ug
201115 Kb DNA Ladder, 250 ug
201190 Salmon Sperm DNA, 10 x 1 ml
201220 QuikHyb Rapid Hybridization Sol,250 ml
201221 QuikHyb Rapid Hybridization Sol,1 L
201222 Kit,MiracleHyb Hybridization Sol,250ml
201223 Kit,MiracleHyb Hybridization Sol,125ml
203003 DNA Ligation Kit
204110 Kit,LipoTAXI Transfection Rgnt 3 x 1 ml
204130 GeneJammer Transfection Reagent, 1 ml
204131 GeneJammer Transfection Reagent,4x1 ml
204132 GeneJammer Transfection Reagent, 0.3 ml
204310 Proteomics grade Trypsin
204525 Kit,Lumi-Max Luminl Enhncd AssySuprxdeA
210513 QC Lightning Multi, 30 Reaction Kit
210514 QC Lightning Multi, 30 rxn Commercial
210515 QC Lightning Multi, 10 Reaction Kit
210516 QC Lightning Multi, 10 rxn Commercial
210518 QuikChange Lightning 10 rxn, -20C
210519 QuikChange Lightning 30 rxn
211170 pCMV-Tag 1 Vector
211172 pCMV-Tag2 Vector
211173 pCMV-Tag3 Vector
211174 pCMV-Tag4 Vector
211175 pCMV-Tag5 Vector
211199 Kit,pCMV-Script PCR Cloning
211203 ExAssist Helper Phage w/ XOLR
211204 Rapid Excision Kit
211521 pET-3 a-d Vectors
211523 pET-11a-d Vectors
211621 Kit,pET-3 a-d Expression system
211623 Kit,pET-11 a-d Expression System
212205 pBluescript II SK(+) Phagemid Kit
212206 pBluescript II SK(-) Phagemid Kit
212207 pBluescript II KS(+) Phagemid Kit
212208 pBluescript II KS(-) Phagemid Kit
212209 pBK-CMV Vector
212215 pBC SK(+) Phagemid
212216 pBC SK(-) Phagemid
212217 pBC KS(+) Phagemid
212218 pBC KS(-) Phagemid
212220 pCMV-Script Vector
212224 pCMV-Script XR Predigested Vector
212240 pBluescript II XR Predigested Vector
212250 pBluescript II RI Predigested Vector
213201 pMC1Neo and pMC1Neo Poly A Vectors
214301 pCAL-c Vector
214302 pCAL-n Vector
214303 Calmodulin Affinity Resin, 10 ml
214310 pCAL-n-EK Vector
214311 pCAL-n-FLAG Vector
214501 pDual Expression Vector
214502 pDual Expression Vector
214503 pDual GC Vector 20 ug (1 ug/ul)
216201 pSG5 Expression Vector
217450 LacSwitch II Mammalian Expression Sys
217451 pESC-HIS Vector
217452 pESC-LEU Vector
217453 pESC-TRP Vector
217454 pESC-URA Vector
217455 pESC Vectors, all four
217460 pERV3 Vector
217461 pEGSH Vector
217467 Ponasterone A, 1 mg
217468 Complete Control Vector Kit
217561 pFB-Neo Vector
217563 pFB Vector
217564 pFB-ERV Vector
217566 pVPack-GP Vector
217567 pVPack-VSV-G Vector
217568 pVPack-Ampho Vector
217569 pVPack-Eco Vector
217570 pVPack-10A1 Vector
219000 PathDetect c-JUN Trans-Reporting System
219001 PathDetect pFR-CAT Trans-Reporter Plsmd
219002 PathDetect pFR-B-Gal Trans-RprtrPlsmd
219004 PathDetect pFR-SEAP Trans-Reportr Plasmd
219005 PathDetect Elk1 Trans-Reporting System
219010 PathDetect CREB Trans-Reporting System
219015 PathDetect CHOP Trans-Reporting System
219020 Luciferase Assay Kit
219026 PathDetect pFA-ATF2 Plasmid
219031 PathDetect pFA-cFos Plasmid
219036 PathDetect pFA-CMV Plasmid
219040 PathDetect pMUT-ELK1 Plasmid
219041 PathDetect pMUT-cJun Plasmid
219042 PathDetect pMUT-CREB Plasmid
219050 PathDetect pFR-Luc Plasmid
219053 PathDetect pFA-Jun Plasmid
219054 PathDetect pFA-CHOP Plasmid
219056 PathDetect pFC-dbd Plasmid
219059 PathDetect pFC-MEKK Plasmid
219062 PathDetect pFA-Elk1 Plasmid
219065 PathDetect pFC-MEK1 Plasmid
219068 PathDetect pFA-CREB Plasmid
219071 PathDetect pFC-PKA Plasmid
219073 PathDetect AP-1 Cis-Reporting System
219074 PathDetect pAP1-Luc Plasmid
219075 PathDetect CRE Cis-Reporting System
219076 PathDetect pCRE-Luc Plasmid
219077 PathDetect NF-kB Cis-Reporting System
219078 PathDetect pNFkB-Luc Plasmid
219079 PathDetect SRE Cis-Reporting System
219080 PathDetect pSRE-Luc Plasmid
219081 PathDetect SRF Cis-Reporting System
219082 PathDetect pSRF-Luc Plasmid
219083 PathDetect p53 Cis-Reporting System
219084 PathDetect pFC-p53 Plasmid
219085 PathDetect p53-Luc Plasmid
219086 PathDetect pFC-MEK3 Plasmid
219087 PathDetect pLuc-MCS Plasmid
219088 PathDetect pNFAT-Luc Plasmid
219089 PathDetect pISRE-Luc Plasmid
219090 PathDetect pCIS-CK Plasmid
219091 PathDetect pGAS-Luc Plasmid
219092 PathDetect ISRE Cis-Reporting System
219093 PathDetect GAS Cis-Reporting System
219094 PathDetect NFAT Cis-Reporting System
219095 PathDetect TARE Cis-Reporting System
230130 BL21-Gold Competent Cells
230132 BL21-Gold (DE3) Competent Cells
230134 BL21-Gold(DE3)pLysS CompCells 10x0.1ml
230191 ArcticExpress Competent Cells
230192 ArcticExpress (DE3) Competent Cells
230193 ArcticExpress (DE3) RIL Competent Cells
230194 ArcticExpress (DE3) RP Competent Cells
230195 ArcticExpress RIL Competent Cells
230196 ArcticExpress RP Competent Cells
230240 BL21-CodonPlus -RIL Competent Cells
230245 BL21-CodonPlus (DE3)-RIL Comp Cells
230246 Protein Expression Competent Cell Pack
230247 Difficult Cloning Competent Cell Pack
230248 Routine Cloning Competent Cell Pack
230250 BL21-CodonPlus -RP Competent Cells
230255 BL21-CodonPlus (DE3)-RP Comp Cells
230265 BL21-CodonPlus (DE3)-RIL-X Comp Cells
230275 BL21-CodonPlus (DE3)-RP-X Comp cells
230280 BL21-CodonPlus (DE3)-RILP Comp Cells
230315 XL10-Gold Bulk Pack - Baylor
230325 SoloPack Gold Competent Cells
230350 SoloPack Gold SuperCompetent Cells
234211 Lambda gt11/EcoR I/CIAP Treated Vctr Kit
234612 Kit,Lambda gt11/EcoR l/Gigapack III Gol
235200 Kit,Lambda CE6 Induction
236201 Lambda ZAP II Vector Kit
236211 Lambda ZAP II/EcoR I/CIAP Treated Vctr
236612 Kit,LambdaZAP II/EcoR l/Gigapack IIIGol
237211 Uni-ZAP XR Vector Kit
237612 Kit,Uni-ZAP /Gigapack III Gold Cloning
239201 ZAP Express Vector Kit
239211 ZAP Express EcoR I/CIAP-Treated Vector
239212 ZAP Express BamH I/CIAP-Treated Vector
239220 Lambda ZAP -CMV Undigested Vector
239221 Lambda ZAP -CMV RI Cloning Kit
239222 Lambda ZAP -CMV XR Cloning Kit
239224 Kit,Lambda ZAP -CMV XR Vector Gigapack
239614 Kit,ZAP Express EcoR II/Gigapack III Vc
239615 Kit,ZAP Express BamH I/Gigapack III Vct
240005 pAdEasy-1 Vector
240006 pShuttle Vector
240007 pShuttle-CMV Vector
240008 pShuttle-CMV lacZ Vector
240009 Kit,AdEasy Adenoviral Vector System
240010 Kit,AdEasy XL Adenoviral System
240028 pCFB-EGSH Retroviral Vector
240029 pFB-Neo-LacZ Retroviral Vector
240030 pFB-Luc Retroviral Vector
240039 PathDetect pTARE-Luc Plasmid
240071 Kit,AAV Helper-Free System
240073 AAV-293 Cells
240081 pShuttle-IRES-hrGFP-1 vector
240085 AD-293 Cells
240099 InterPlay TAP Purification Buffer Kit
240101 InterPlay N-terminal Mammalian TAP Vectr
240102 InterPlay C-Terminal Mammalian TAP Vectr
240103 Kit,InterPlay N-Terminal MammalianTAPSy
240104 Kit,InterPlay C-TerminalMammalianTAP Sy
240105 Streptavidin Resin 2.5 ml
240106 MS-Grade Calmodulin Resin 0.625 ml
240107 InterPlay Mammalian TAP Purfication Kit
240109 AAV-HT1080 Cells
240111 PathDetect c/EBP Cis-Reporting System
240112 PathDetect c/EBP Cis-Reporting Plasmid
240113 PathDetect DR1/RXRE Cis-Reporting System
240114 PathDetect DR1/RXRE Cis-Reporting Plasmd
240115 PathDetect DR3/VDR Cis-Reporting System
240116 PathDetect DR3/VDR Cis-Reporting Plasmd
240119 PathDetect DR5/RARE Cis-Reporting System
240120 PathDetect DR5/RARE Cis-Reporting Plasmd
240129 PathDetect Egr-1 Cis-Reporting System
240130 PathDetect Egr-1 Cis-Reporting Plasmid
240131 PathDetect LILRE Cis-Reporting System
240132 PathDetect LILRE Cis-Reporting Plasmid
240133 PathDetect GRE Cis-Reporting System
240134 PathDetect GRE Cis-Reporting Plasmid
240135 PathDetect DR4/TR Cis-Reporting System
240136 PathDetect DR4/TR Cis-Reporting Plasmid
240141 Vitality full length hrGFPantibdy,20ul
240162 VeriFlex N-terminal SBP Vector Set
240163 Kit,VariFlex N-terminal SBP System
240164 VariFlex N-terminal SBP-SET Vector Set
240165 Kit,VariFlex N-terminalSBP-SETExprssnSy
240172 VariFlex N-Terminal SET Vector Set
240174 VariFlex C-Terminal SBP Vector
240175 VariFlex C-Terminal SBP System
240176 VariFlex C-Terminal SBP-SET Vector Set
240177 VariFlex C-terminal SBP-SET Expressn Sys
240184 VariFlex C-terminal SET Vector Set
240195 pCMV-3Tag-1 Epitope TaggingMammlnExpVctr
240196 pCMV-3Tag-2 Epitope TaggingMammlnExpVctr
240197 pCMV-3Tag-3 Epitope TaggingMammlnExpVctr
240198 pCMV-3Tag-4 Epitope TaggingMammlnExpVctr
240200 pCMV-3Tag-6 vectors
240202 pCMV-3Tag-7 vectors
240203 pCMV-3Tag-8 vectors
240204 pCMV-3Tag-9 vectors
240205 StrataClone PCR Cloning Kit
240207 StrataClone Blunt PCR Cloning Kit
240213 InterPlay Adenoviral N-Terminal TAP Sys
240214 InterPlay Adenoviral N-terminal TAP Vctr
240215 InterPlay Adenoviral C-Terminal TAP Sys
240216 InterPlay Adenoviral C-Terminal Vectors
240218 StrataClone Ultra Blunt PCR Cloning Kit
240228 StrataClone Mammalian Untagged
240229 StrataClone Mammalian N-Flag
240230 StrataClone Mammalian C-Flag
240231 StrataClone Mammalian N-cMyc
240232 StrataClone Mammalian C-cMyc
240241 Vitality hrGFP monclonal antibody 20 ul
240242 Vitality hrGFP monoclonal antibody 100ul
240243 AdEasy Virus Purification Kit 2x100
241211 Lambda EMBL3/BamH I Vector Kit
241612 Kt,LambdaEMBL3/Gigapack III Gold Clnin
241711 Kt,Lambda EMBL3/Gigapack II XL Clonin
247201 Lambda DASH II Undigested Vector Kit
247211 Lambda DASH II/BamH I Vector Kit
247212 Lambda DASH II/EcoR I Vector Kit
247613 Kt,Lambda DASH II/BamH I/Gigapack III
247614 Kit,Lambda DASH II/EcoR I/Gigapack III
247713 Kit,LambdaDASH II/BamHI/Gigapack III X
247714 Kit,LambdaDASH II/EcoRI/Gigapack III XL
248201 Lambda FIX II Undigested Vector Kit
248211 Lambda FIX II/Xho I Partial Fill-in Vect

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